Open AccessStructure of secondary cleavage sites ofE. coliRNAaseIII in A3t RNA from bacteriophage T7
Author(s) -
Gerhard Groß,
John J. Dunn
Publication year - 1987
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/15.2.431
Subject(s) - biology , cleavage (geology) , rna , library science , bacteriophage , nucleic acid , escherichia coli , genetics , gene , paleontology , computer science , fracture (geology)
Five 'secondary' cleavage sites of E. coli RNAaseIII within the initiator RNA A3t (141 nucleotides) from bacteriophage T7 are described. Cleavage takes place apparEntly within or at the bottom of a short double-stranded stretch of RNA. Three secondary sites are efficiently cleaved in the presence of magnesium 2+ ions, two additional sites are cleaved in the presence of manganese 2+ ions at low monovalent salt concentrations (less than 0.05 M).