Open AccessIsolation and characterization of the positively acting regulatory geneQUTAfromAspergillus nidulans
Author(s) -
Raj K. Beri,
Hayley A. Whittington,
C. F. Roberts,
Alastair R. Hawkins
Publication year - 1987
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/15.19.7991
Subject(s) - biology , aspergillus nidulans , gene , genetics , open reading frame , dna , homology (biology) , regulator gene , gene cluster , neurospora crassa , sequence analysis , nucleic acid sequence , regulatory sequence , peptide sequence , microbiology and biotechnology , regulation of gene expression , mutant
The positively acting regulator gene QUTA from Aspergillus nidulans has been identified and located within a cluster of quinic acid utilisation (QUT) genes isolated within a recombinant phage lambda (lambda Q1). The DNA sequence of the QUTA gene reveals a single uninterrupted reading frame coding for a protein of mw 90.416 Kd. The QUTA protein sequence has a protein motif in the form of a putative "DNA finger" that shows strong homology to other such motifs in the GAL4, PPR1, ARGRII, LAC9 and QA1F regulatory gene products of S. cerevisiae, K. lactis and N. crassa. The data presented confirm the view deduced by genetical analysis that the QUTA gene of A. nidulans encodes a protein capable of interacting with QUT specific DNA sequences.