The nucleotide sequence of thePRIIgene related to DNA primase inSaccharomyces cerevisiae | Zendy

Paolo Picvani | Zendy; Stefania Francesconi | Zendy; G. Lucchini | Zendy

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The nucleotide sequence of thePRIIgene related to DNA primase inSaccharomyces cerevisiae

Author(s) -

Paolo Picvani,

Stefania Francesconi,

G. Lucchini

Publication year - 1987

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/15.19.7975

Subject(s) - biology , genetics , gene , primase , saccharomyces cerevisiae , humanities , polymerase chain reaction , philosophy , reverse transcriptase

The PRI1 gene of Saccharomyces cerevisiae encodes for the p48 polypeptide of DNA primase. We have determined the nucleotide sequence of a 1,965 bp DNA fragment containing the PRI1 locus. The entire coding sequence of the gene lies within an open reading frame, and there are 409 amino acids in the single polypeptide protein if translation is assumed to start at the first ATG in this frame. The 5' and 3' end-points of PRI1 mRNA have been determined by S1 mapping and primer extension analysis. The primary structure and the codon usage of PRI1 suggest that this essential gene is poorly expressed in yeast cells.

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