Open AccessThe nucleotide sequence of thePRIIgene related to DNA primase inSaccharomyces cerevisiae
Author(s) -
Paolo Plevani,
Stefania Francesconi,
Giovanna Lucchini
Publication year - 1987
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/15.19.7975
Subject(s) - biology , primase , saccharomyces cerevisiae , genetics , nucleic acid sequence , gene , dna , nucleotide , microbiology and biotechnology , polymerase chain reaction , reverse transcriptase
The PRI1 gene of Saccharomyces cerevisiae encodes for the p48 polypeptide of DNA primase. We have determined the nucleotide sequence of a 1,965 bp DNA fragment containing the PRI1 locus. The entire coding sequence of the gene lies within an open reading frame, and there are 409 amino acids in the single polypeptide protein if translation is assumed to start at the first ATG in this frame. The 5' and 3' end-points of PRI1 mRNA have been determined by S1 mapping and primer extension analysis. The primary structure and the codon usage of PRI1 suggest that this essential gene is poorly expressed in yeast cells.