Open AccessEuglena gracilischloroplast DNA: the untranslated leader oftufA-ORF206 gene contains an intron
Author(s) -
Paul-Etienne Montandon,
Colette Knuchel-Aegerter,
Erhard Stutz
Publication year - 1987
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/15.19.7809
Subject(s) - biology , intron , euglena gracilis , chloroplast dna , genetics , coding region , rna splicing , group ii intron , untranslated region , gene , ribosome , tufa , start codon , stop codon , five prime untranslated region , microbiology and biotechnology , messenger rna , chloroplast , rna , materials science , carbonate , metallurgy
Structural features of a dicistronic 1.95 kb mRNA coding for the chloroplast specific elongation factor Tu and ORF206 are described. The unspliced pre-mRNA is composed of 2562 nucleotides and undergoes four splicing events which remove a total of 606 nucleotides. The first intron splits the untranslated leader, two introns dissect the tufA coding region and the forth intron is within ORF206, which codes for a putative protein that is to 34% homologous with the putative protein of chloroplast ORF184 of tobacco. Introns neither belong to group I nor II, and 5' and 3' intron boundaries do not follow consensus sequences. Potential ribosome binding sites are located 58 and 32 positions upstream of the tufA and ORF206 start codon, respectively.