Hybridization properties of immobilized nucleic acids | Zendy

T Gingeras | Zendy; Deborah Y. Kwoh | Zendy; G. R. Davis | Zendy

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Hybridization properties of immobilized nucleic acids

Author(s) -

T Gingeras,

Deborah Y. Kwoh,

G. R. Davis

Publication year - 1987

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/15.13.5373

Subject(s) - oligonucleotide , biology , nucleic acid thermodynamics , nucleic acid , in situ hybridization , nitrocellulose , microbiology and biotechnology , biochemistry , conjugate , oligomer restriction , sequencing by hybridization , dna–dna hybridization , dna , gene , messenger rna , base sequence , dna sequencing , membrane , mathematical analysis , mathematics , dna sequencer

The 5'-end attachment of oligonucleotides to dextran supports facilitates the study of the hybridization properties of an immobilized oligonucleotide system. The hybridization properties which were studied include: hybridization capacity and kinetics, hybridization-complex stability, and reagents influencing hybridization efficiency. Results of these experiments reveal that the hybridization efficiencies of support-bound oligonucleotides were 75-80% and 40-50% for single-stranded oligonucleotide targets and long double-stranded targets, respectively. These hybridization efficiencies are dependent upon prehybridizing the support-bound oligonucleotides with dextran sulfate. In addition, comparisons of the relative hybridization efficiencies of the support-bound oligonucleotide and nitrocellulose-based systems have been made which indicate a retention of 13-28% of target sequences on the filters and a detection efficiency of 8-20%.

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