Nucleotide sequence of an heterochromatic segment recognized by the antibodies to Z-DNA in fixed metaphase chromosomes | Zendy

Bernard Malfoy | Zendy; N. Rousseau | Zendy; Nicolas Vogt | Zendy; E. Viégas-Pèquignot | Zendy; Bernard Dutrillaux | Zendy; M. Leng | Zendy

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Nucleotide sequence of an heterochromatic segment recognized by the antibodies to Z-DNA in fixed metaphase chromosomes

Author(s) -

Bernard Malfoy,

N. Rousseau,

Nicolas Vogt,

E. Viégas-Pèquignot,

Bernard Dutrillaux,

M. Leng

Publication year - 1986

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/14.8.3197

Subject(s) - biology , heterochromatin , dna , nucleic acid sequence , metaphase , satellite dna , microbiology and biotechnology , base pair , z dna , genetics , sequence (biology) , repeated sequence , chromosome , dna sequencing , gene , genome , chromatin

The purpose of this work was to analyse at the molecular level the DNA recognized by the antibodies to Z-DNA in in situ experiments. Antibodies to Z-DNA interact strongly with R-band positive heterochromatic segments of fixed metaphase chromosomes of Cebus (Viegas-Pequignot et al., 1983). These segments are constituted of a satellite DNA the repeat unit of which is about 1520 base pairs long. The base sequence of the repeat unit has been determined. It contains a (AC)n rich region which, in vitro, adopts the Z conformation under topological constraints. Experiments with nuclei suggest that this sequence is not predominantly in the Z conformation in vivo. The polymorphic structure of the (AC)n rich region argues for an active recombination sequence.

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