Open AccessMolecular cloning and sequence analysis of human Na, K-ATPase β–subunit
Author(s) -
Kiyoshi Kawakami,
Hiroshi Nishimura,
Toshiko Ohta,
Kei Nagano
Publication year - 1986
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/14.7.2833
Subject(s) - biology , microbiology and biotechnology , complementary dna , nucleic acid sequence , coding region , peptide sequence , southern blot , sequence analysis , protein subunit , northern blot , homology (biology) , cdna library , genomic dna , molecular cloning , genomic library , gene , genetics
We have isolated a cDNA clone for the beta-subunit of HeLa cell Na,K-ATPase, containing a 2208-base-pair cDNA insert covering the whole coding region of the beta-subunit. Nucleotide sequence analysis revealed that the amino acid sequence of human Na,K-ATPase exhibited 61% homology with that of Torpedo counterpart (Noguchi et al. (1986) FEBS Lett. in press). A remarkable conservation in the nucleotide sequence of the 3' non-coding region was detected between the human and Torpedo cDNAs. RNA blot hybridization analysis revealed the presence of two mRNA species in HeLa cells. S1 nuclease mapping indicated that they were derived from utilization of two distinct polyadenylation signals in vivo. Total genomic Southern hybridization indicated the existence of only a few, possibly one set of gene encoding the Na,K-ATPase beta-subunit in the human genome.