Open AccessNucleotide sequence analysis of the spacer regions flanking the rat rRNA transcription unit and identification of repetitive dements
Author(s) -
Libert Yavachev,
O.I. Georgiev,
Э. А. Брага,
T.A. Avdonina,
Anastasiya E. Bogomolova,
Victor B. Zhurkin,
В. В. Носиков,
A.A. Hadjiolov
Publication year - 1986
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/14.6.2799
Subject(s) - biology , nucleic acid sequence , genetics , 5' flanking region , identification (biology) , transcription (linguistics) , nucleotide , sequence (biology) , microbiology and biotechnology , computational biology , gene , gene expression , promoter , linguistics , philosophy , botany
We investigated the organization of the rat rDNA non-transcribed spacer (NTS) by determining the sequence of large NTS segments located upstream (2501 bp) and downstream (4025 bp) from the rRNA transcription unit. We identified four B2-like and two ID mobile elements. They may be grouped in three pairs with the members of each pair located in the upstream and downstream NTS. The ID sequences are identical to the consensus sequence, while the pairs of B2-like elements show 85% and 50/65% homology to the consensus B2 sequence. The proximal part of the downstream NTS contains a region of widely diverged SalI tandem repeats. A considerable part of the analyzed upstream and downstream NTS sequences is constituted by different types of simple sequences and long poly(purine) X poly(pyrimidine) tracts. These data show that the rat rDNA NTS regions flanking the rRNA transcription unit are characterized by a combination of short interspersed (B2-superfamily) and various simple sequences.