Open AccessNucleotide sequence and expression of aPseudomonas savastanoicytokinin biosynthetic gene: homotogy withAgrobacterium tumefaciens tmrandtzsloci
Author(s) -
Gary K. Powell,
Roy O. Morris
Publication year - 1986
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/14.6.2555
Subject(s) - biology , agrobacterium tumefaciens , ti plasmid , agrobacterium , nucleic acid sequence , gene , genetics , plasmid , pseudomonas syringae , open reading frame , pseudomonas , peptide sequence , homology (biology) , xanthomonas campestris , shuttle vector , octopine , microbiology and biotechnology , bacteria , transformation (genetics) , vector (molecular biology) , recombinant dna
The nucleotide sequence of a Pseudomonas trans-zeatin producing gene (ptz) from the pCK1 plasmid of Pseudomonas syringae pv. savastanoi strain 1006 has been determined. This gene confers upon E. coli the ability to synthesize and secrete several cytokinins including trans-zeatin, iso-pentenyladenine and their respective N9-ribosyl derivatives. Sequence analysis indicates an open reading frame encoding a protein of 234 amino acids with a molecular weight of 26,816. Significant sequence homology is found between ptz and both the tzs and tmr genes from Agrobacterium tumefaciens. The results suggest a close relationship between the cytokinin biosynthetic pathways in P. savastanoi and A. tumefaciens.