Open AccessmRNA transcripts of several plant genes are polyadenylated at multiple sitesin vivo
Author(s) -
Caroline Dean,
Stanley Tamaki,
Pamela Dunsmuir,
Mitchell Favreau,
Carol D. Katayama,
Hugo K. Dooner,
John Bedbrook
Publication year - 1986
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/14.5.2229
Subject(s) - polyadenylation , biology , gene , complementary dna , genetics , terminator (solar) , microbiology and biotechnology , rubisco , untranslated region , sequence analysis , gene expression , messenger rna , ionosphere , physics , astronomy
We have analyzed the polyadenylation sites for the small subunit of ribulose bisphosphate carboxylase and chlorophyll a/b binding protein genes of Petunia (Mitchell) and the bronze gene of Zea mays. Sequence analysis of multiple cDNA clones revealed that polyadenylation of the transcripts occurred at either 2 or 3 sites for all three groups of genes. In the examples where 3 polyadenylation sites were detected, the middle site was the one predominantly used. Putative polyadenylation signals preceding the poly A tails diverged significantly from the animal consensus sequence AATAAA. In all the genes examined the first A residue in the poly A tail of the cDNA clones corresponded to an A residue in the homologous genomic sequence.