Open AccessTwo unique restriction endonucleases fromNeisseria lactamica
Author(s) -
Boqin Qiang,
Ira Schildkraut
Publication year - 1986
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/14.5.1991
Subject(s) - biology , neisseria , sequence (biology) , restriction enzyme , genetics , peptide sequence , microbiology and biotechnology , bacteria , dna , gene
Two new site-specific endonucleases, N1a III and N1a IV, have been isolated from Neisseria lactamica. N1a III recognizes the sequence, CATG, and cleaves 3' of the sequence to produce a four base 3' extension. N1a IV recognizes the sequence, GGNNCC, and cleaves between the two N's to produce blunt ended fragments.