Open AccessIsolation of a further anonymous informative DNA sequence from chromosome seven closely linked to cystic flbrosis
Author(s) -
Peter J. Scambler,
Brandon Wainwright,
E. Walson,
Gillian P. Bates,
G. Bell,
Robert Williamson,
Martin Farrall
Publication year - 1986
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/14.5.1951
Subject(s) - biology , genetics , locus (genetics) , ecori , allele , chromosome , genetic marker , cystic fibrosis , restriction fragment length polymorphism , dna , gene , restriction enzyme , genotype
A library prepared from flow-sorted chromosomes was used to isolate single-copy sequences from chromosome seven. One such sequence 7C22 has been shown to be polymorphic for an EcoRI restriction site and to be informative for the study of CF in approximately 35% of matings. The segregation of the 7C22 alleles was followed through nineteen informative families with more than one child affected by cystic fibrosis. We report that the locus for 7C22 is linked to the locus for cystic fibrosis at a recombination fraction of 0.045. This marker will prove useful in improving the accuracy and informativeness of prenatal diagnosis and in constructing a fine genetic map around the cystic fibrosis gene.