Open AccessNudeotide sequence of the chicken 5-aminolevulinate synthase gene
Author(s) -
Deborah Maguire,
Adrienne R. Day,
Iain A. Borthwick,
Gopesh Srivastava,
Peter L. Wigley,
Brian K. May,
William H. Elliott
Publication year - 1986
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/14.3.1379
Subject(s) - biology , exon , gene , atp synthase , microbiology and biotechnology , primer extension , nucleic acid sequence , heme , genetics , enzyme , nucleotide , biochemistry
5-Aminolevulinate synthase, the first and rate-controlling enzyme of heme biosynthesis, is regulated in the liver by the end-product heme. To study this negative control mechanism, we have isolated the chicken gene for ALA-synthase and determined the nucleotide sequence. The structural gene is 6.9 kb long and contains 10 exons. The transcriptional start site for ALA-synthase was determined by primer extension analysis. A fragment of 291 bp from the 5' flanking region including 34 bp of the first exon shows promoter activity when introduced upstream of a chicken histone H2B gene and injected into the nuclei of Xenopus laevis oocytes.