Open AccessSynthesis of viral DNA forms inNicotiana plumbaginifoliaprotoplasts inoculated with cassava latent virus (CLV); evidence for the independent replication of one component of the CLV genome
Author(s) -
R. Townsend,
J. W. Watts,
John Stanley
Publication year - 1986
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/14.3.1253
Subject(s) - biology , genome , dna , virology , virus , viral replication , genomic dna , genetics , microbiology and biotechnology , gene
Totipotent leaf mesophyll protoplasts of Nicotiana plumbaginifolia, Viviani were inoculated with cassava latent virus (CLV) or with full length copies of CLV genomic DNAs 1 and 2 excised from replicative forms of M13 clones. Virus specific DNAs began to appear 48-72h after inoculation with virus or cloned DNAs, coincident with the onset of host cell division. Infected cells accumulated supercoiled forms of DNAs 1 and 2 as well as progeny single-stranded (ss) virion (+) sense DNAs representing each component of the genome. Both supercoiled and ss molecules were synthesised by cells inoculated with cloned DNA 1 alone but DNA 2 failed to replicate independently.