Open AccessNuclotide sequence and organization ofdnaBgene and neighbouring genes on theBacillus subtilischromosome
Author(s) -
Nagahisa Ogasawara,
Shigeki Moriya,
P Mazza,
Hiroshi Yoshikawa
Publication year - 1986
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/14.24.9989
Subject(s) - biology , bacillus subtilis , gene , genetics , orfs , open reading frame , nucleic acid sequence , start codon , dna , microbiology and biotechnology , peptide sequence , nucleotide , bacteria
A region of the Bacillus subtilis chromosome containing dnaB, a gene essential for the initiation of chromosomal replication in B. subtilis, was cloned and nucleotide sequence of some 5000bp determined. The region consists of 4 open reading frames (ORFs) possibly comprising a single transcriptional unit. Two dnaB mutations, dnaB27 and dnaB19 were located within the first ORF which would give rise to a protein of 472 amino acids. The dnaB27 mutation involves codon at the position of 122 (replacement of Asp by Asn) close to a DNA binding domain and the dnaB19 codon 379 (replacement of Ala by Thr) close to a region rich in charged amino acids which may be alpha helical. The third ORF in the same transcriptional unit would produce a hydrophobic protein which might be involved in the DNA-membrane binding function of dnaB gene. No homologous Escherichia coli genes have been found.