Open AccessNucleotide sequence of the virulence genevirGof theAgrobacterium tumefaciensoctopine Ti plasmid: significant homology betweenvirGand the regulatory genesompR,phoBanddyeofE. coli
Author(s) -
Leo S. Melchers,
David V. Thompson,
Ken B. Idler,
R. A. Schilperoort,
Paul J. J. Hooykaas
Publication year - 1986
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/14.24.9933
Subject(s) - octopine , biology , ti plasmid , agrobacterium tumefaciens , genetics , plasmid , gene , agrobacterium , nucleic acid sequence , regulator gene , t dna binary system , homology (biology) , open reading frame , microbiology and biotechnology , peptide sequence , regulation of gene expression , transformation (genetics) , vector (molecular biology) , recombinant dna
The entire nucleotide sequence of the virG locus, from the octopine Ti plasmid of Agrobacterium tumefaciens strain 15955, has been determined. The virG gene is 801 nucleotides in length and has one open reading frame which encodes a protein of Mr 29,995. The virG gene is involved in the transcriptional activation of the Ti plasmid vir-loci, which occurs after induction by specific compounds present in plant exudate. Sequence analysis of the Agrobacterium virG protein showed significant homology with the Escherichia coli ompR, phoB and dye proteins, which are all positive regulatory genes for genes encoding envelope proteins. These results suggest that the virG gene encodes a positive regulatory protein which can activate vir gene expression.