A bacterial protein requirement for the bacteriophage λ terminase reaction | Zendy

Marvin Gold | Zendy; Wendy Parris | Zendy

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A bacterial protein requirement for the bacteriophage λ terminase reaction

Author(s) -

Marvin Gold,

Wendy Parris

Publication year - 1986

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/14.24.9797

Subject(s) - biology , bacteriophage , siphoviridae , bacterial protein , bacteria , microbiology and biotechnology , virology , genetics , escherichia coli , gene

The bacteriophage lambda terminase enzyme cleaves the cohesive-end sites of lambda DNA to yield the protruding 5'-termini of the mature molecule. In vitro, this endonucleolytic event requires a protein factor which has been isolated and purified from extracts of uninfected E. coli. The terminase host factor (THF) is a heat stable basic protein of M.W. approximately 22,000. The integration host factor (IHF) protein of E. coli can efficiently substitute for THF in the terminase reaction; however, THF can be demonstrated to be physically present in, and isolated with full biological activity from extracts of cells defective or deficient in IHF.

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