Open AccessNucleotide sequence ofBacillusphage Ø29 genes 14 and 15: homology of gene 15 with other phage lysozymes
Author(s) -
Kevin J. Garvey,
Mohammad S. Saedi,
Junetsu Ito
Publication year - 1986
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/14.24.10001
Subject(s) - biology , homology (biology) , nucleic acid sequence , gene , open reading frame , genetics , microbiology and biotechnology , mutant , molecular cloning , peptide sequence , escherichia coli , bacteriophage
The nucleotide sequence of Bacillus phage phi 29 genes 14 (g14) and 15 (g15) have been determined and shown to encode proteins with molecular weights of 15,014 and 28,022, respectively. The g14 open reading frame (ORF) was confirmed by sequencing a sus14(1241) mutant. Gene product 15 (gp15) has considerable homology with Salmonella phage P22 lysozyme and lesser homology with Escherichia coli phage T4 lysozyme. Putative translation signals are identified. In addition, the role of a previously described promoter, B2, is discussed.