Isolation of a polymorphic DNA sequence (λEMBL3.121, D14S12) from chromosome 14 | Zendy

Jennifer Brennan | Zendy; N.K. Spurr | Zendy; Walter F. Bodmer | Zendy

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Isolation of a polymorphic DNA sequence (λEMBL3.121, D14S12) from chromosome 14

Author(s) -

Jennifer Brennan,

N.K. Spurr,

Walter F. Bodmer

Publication year - 1986

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/14.22.9225

Subject(s) - biology , isolation (microbiology) , library science , chromosome , genetics , gene , bioinformatics , computer science

SOURCE/DESCRIPTION: 14kb single copy clone in EMBL3 from a complete BamHl digest of DNA from the human lymphoblastoid cell line, Mann. POLYMORPHISM: Bglll (A/GATCT) (Anglian Biotechnology Ltd.) identified a two allele polymorphism with bands at either 9kb, 4.4kb, 2.6kb 2.3kb (Al) or 9kb, 7.2kb, 2.3kb (A2) FREQUENCY: Studied 30 European Whites, Al allele 0.63 A2 allele 0.37 NOT POLYMORPHIC FOR: , Taql, Mspl CHROMOSOMAL LOCALISATION: p robe assigned to chr0 mosome 14 using a panel of human-rodent somatic cell hybrids. MENDELIAN INHERITANCE: Co-dominant segregation demonstrated in one family (13 individuals) OTHER COMMENTS: RFLPs were observed under normal stringency (0.2xSSC) ACKNOWLEDGEMENTS: We would like to thank Dr. E. Solomon (ICRF) and S. Povey (MRC Biochemical Genetics Units, London) for DNA samples from somatic cell hybrids.

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