Cloning of cDNA for human T-cell replacing factor (interieukin-5) and comparison with the murine homologue | Zendy

Chihiro Azuma | Zendy; Toshizumi Tanabe | Zendy; Mikdo Konishi | Zendy; Tatsuo Kinashi | Zendy; Takafumi Noma | Zendy; Fumihiko Matsuda | Zendy; Yoshio Yaoita | Zendy; Kiyoshi Takatsu | Zendy; Lennart Hammarström | Zendy; Smith Rjh | Zendy; Eva Severinson | Zendy; Tasuku Honjo | Zendy

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Cloning of cDNA for human T-cell replacing factor (interieukin-5) and comparison with the murine homologue

Author(s) -

Chihiro Azuma,

Toshizumi Tanabe,

Mikdo Konishi,

Tatsuo Kinashi,

Takafumi Noma,

Fumihiko Matsuda,

Yoshio Yaoita,

Kiyoshi Takatsu,

Lennart Hammarström,

Smith Rjh,

Eva Severinson,

Tasuku Honjo

Publication year - 1986

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/14.22.9149

Subject(s) - complementary dna , biology , microbiology and biotechnology , nucleic acid sequence , peptide sequence , cdna library , coding region , molecular cloning , signal peptide , amino acid , cloning (programming) , gene , biochemistry , computer science , programming language

We have cloned cDNA for T-cell replacing factor (interleukin-5), which replaces T-cell helper function for normal B cells which secrete immunoglobulin, from human T cell leukemia line, ATL-2, using mouse interleukin-5 cDNA as probe. Total nucleotide sequence of the cDNA (816 base pairs) was determined and compared with that of mouse interleukin-5 cDNA. The cloned cDNA encoded the interleukin-5 precursor of 134 amino acids containing an N-terminal signal sequence. Although the human interleukin-5 precursor is one amino acid longer than the murine homologue, the sizes of the mature proteins appear similar. The nucleotide and amino acid sequence homologies of the coding regions of human and murine interleukin-5 are 77% and 70%, respectively. Human interleukin-5 synthesized by the direction of the cloned cDNA induced immunoglobulin synthesis in human B cells stimulated by Staphylococcus aureus mitogen.

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