Construction of a retroviral cDNA version of the int-l mammary oncogene and its expression in vitro | Zendy

F.A.M. Rijsewijk | Zendy; Maarten van Lohuizen | Zendy; Albert van Ooyen | Zendy; Roel Nusse | Zendy

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Construction of a retroviral cDNA version of the int-l mammary oncogene and its expression in vitro

Author(s) -

F.A.M. Rijsewijk,

Maarten van Lohuizen,

Albert van Ooyen,

Roel Nusse

Publication year - 1986

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/14.2.693

Subject(s) - library science , biology , oncogene , cancer , classics , history , genetics , computer science , cell cycle

The int-1 mammary oncogene is activated by proviruses of the Mouse Mammary Tumor Virus in many different mammary tumors. We have inserted a genomic fragment containing the protein-encoding domain of the gene into the retroviral shuttle vector pZIPneoSV(X)1. After one round of virus replication we recovered recombinant proviral DNA containing a correctly spliced copy of int-1. In vitro transcription of this cDNA version of int-1 using SP6 polymerase and translation in a reticulocyte lysate yielded a protein of approximately 37,000 daltons. High expression of int-1 in NIH-3T3 cells infected with recombinant virus did not lead to morphological transformation.

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