Open AccessHybridization triggered cross-linking of deoxyoligonucleotides
Author(s) -
Thomas R. Webb,
Mark D. Matteucci
Publication year - 1986
Publication title -
nucleic acids research
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/14.19.7661
Subject(s) - oligonucleotide , biology , combinatorial chemistry , base (topology) , protecting group , biochemistry , base sequence , oligonucleotide synthesis , dna , organic chemistry , chemistry , mathematical analysis , alkyl , mathematics
This paper reports details of the synthesis of oligodeoxynucleotides containing the modified base 5-methyl-N4,N4-ethanocytosine (Ce). The 9-fluorenylmethoxycarbonyl group is used as a protecting group for the exocyclic amines of dA and dC. This group can be removed rapidly under very mild conditions. Oligomers containing the Ce base form a cross-link when hybridized to their complementary deoxyoligonucleotides. Some of the scope and limitations of these cross-link forming oligonucleotides are reported.