Hybridization triggered cross-linking of deoxyoligonucleotides | Zendy

Thomas R. Webb | Zendy; Mark D. Matteucci | Zendy

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Hybridization triggered cross-linking of deoxyoligonucleotides

Author(s) -

Thomas R. Webb,

Mark D. Matteucci

Publication year - 1986

Publication title -

nucleic acids research

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/14.19.7661

Subject(s) - oligonucleotide , biology , combinatorial chemistry , base (topology) , protecting group , biochemistry , base sequence , oligonucleotide synthesis , dna , organic chemistry , chemistry , mathematical analysis , alkyl , mathematics

This paper reports details of the synthesis of oligodeoxynucleotides containing the modified base 5-methyl-N4,N4-ethanocytosine (Ce). The 9-fluorenylmethoxycarbonyl group is used as a protecting group for the exocyclic amines of dA and dC. This group can be removed rapidly under very mild conditions. Oligomers containing the Ce base form a cross-link when hybridized to their complementary deoxyoligonucleotides. Some of the scope and limitations of these cross-link forming oligonucleotides are reported.

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