The core promoter of mouse rDNA consists of two functionally distinct domains | Zendy

Joachim Clos | Zendy; Andrea Normann | Zendy; Andrea Öhrlein | Zendy; Ingrid Grummt | Zendy

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The core promoter of mouse rDNA consists of two functionally distinct domains

Author(s) -

Joachim Clos,

Andrea Normann,

Andrea Öhrlein,

Ingrid Grummt

Publication year - 1986

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/14.19.7581

Subject(s) - biology , transcription (linguistics) , promoter , transcription factor , upstream activating sequence , response element , point mutation , genetics , binding site , e box , microbiology and biotechnology , sequence motif , transcription factor ii e , enhancer , gene , transcriptional regulation , mutant , gene expression , philosophy , linguistics

We have determined the sequences constituting the minimal promoter of mouse rDNA. A very small region immediately upstream of the transcription start site (from -1 to -39) is sufficient to direct correct transcription initiation. Sequences immediately downstream of the transcription start site (+1 to +11) increase the efficiency of transcription initiation. Point mutations within the core promoter have been generated and assayed for their effects on template activity and on interaction with the pol I specific transcription factor TIF-IB. The core promoter element appears to consist of two functionally different domains. The distal sequence motif from position -22 to -16 is recognized by factor TIF-IB. Mutations within this region lead to similar changes of both template activity and binding of TIF-IB. Two point mutations within the proximal sequence motif from -15 to -1 do not affect TIF-IB binding although they severely impair transcription initiation. It is suggested, that this proximal region plays a role in the assembly of functional transcription initiation complexes rather than in the primary binding of TIF-IB.

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