Nucleotide sequence of pre-pro-von Willebrand factor cDNA

This cDNA sequence is complete except for approximately 150 bp of 5' untranslated and lOObp of 3 untranslated sequence. The whole sequence was derived on both strands. An upstream stop codon in the major open reading frame is shown by asterisks. Potential sites for N-linked glycosylation are underlined. The sites of cleavage of signal peptide and vWF pro-polypeptide (1) are indicated by arrows. The sequence ArgGlyAspSer (2) is boxed. Araino-acids are numbered to the right, relative to the initiator methionine as 1. The clones analysed were isolated from a human umbilical vein endothelial cell cDNA library (3). The repeated elements within this sequence will be discussed elsewhere (4).