Open AccessNucleotide sequence of the gene responsible for D-xylose uptake inEscherichia coli
Author(s) -
Naotaka Kurose,
Kunihiko Watanabe,
Akira Kimura
Publication year - 1986
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/14.17.7115
Subject(s) - biology , nucleic acid sequence , start codon , genetics , open reading frame , escherichia coli , coding region , gene , microbiology and biotechnology , conserved sequence , stop codon , peptide sequence , sequence (biology) , inverted repeat , consensus sequence , nucleotide , genome
The nucleotide sequence of the cloned DNA, 363 bp in length, has been determined. It can complement the mutation of Escherichia coli having a decreased activity of D-xylose uptake at low temperature. Nucleotide sequence analysis found one possible reading frame coding for a polypeptide consisting of 61 amino acids. Several signal sequences conserved in the promoter regions of E. coli were found in the upstream regions of the open frame. This included the Shine-Dalgarno sequence, the Pribnow box, and the sequence conserved in the "-35 region" with a preferable spacing from each other for an efficient transcription. Downstream from the termination codon, the inverted repeat sequence was present, followed by 3 successive T's.