Open AccessTheTRP4gene ofSaccharomyces cerevisiae: isolation and structural analysis
Author(s) -
Rolf Furter,
Gerhard Paravicini,
Markus Aebi,
Gerhard H. Braus,
Franziska Prantl,
Peter Niederberger,
Ralf Hütter
Publication year - 1986
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/14.16.6357
Subject(s) - biology , saccharomyces cerevisiae , isolation (microbiology) , genetics , gene , saccharomyces , computational biology , microbiology and biotechnology
The TRP4 gene of Saccharomyces cerevisiae, encoding the anthranilate phosphoribosyl transferase, was isolated and subcloned by functional complementation in yeast. A 2 kb fragment containing information for a polypeptide of 380 amino acids and the 5'- and 3'-flanking regions was sequenced. The TRP4 transcript was identified and mapped with S1 nuclease. Homologies to two prokaryotic genes encoding the same function, and sequences potentially involved in transcription start and termination and in regulation of TRP4 gene expression are discussed.