Open AccessUse of a 1- hydrorybenzotriazole activated pbospborylating reagent towards the synthesis of short RNA fragments in solution
Author(s) -
Erik de Vroom,
A. Fidder,
J. E. Marugg,
G.A. van der Marel,
van J.H. Boom
Publication year - 1986
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/14.14.5885
Subject(s) - guanine , reagent , biology , rna , uracil , combinatorial chemistry , nucleic acid , biochemistry , nucleotide , dna , organic chemistry , chemistry , gene
Evidence will be presented to show that the reagents 3a-c (X = O) obtained by the reaction of 2-chlorophenylphosphorodichloridate with 1-hydroxybenzotriazole or 1-hydroxy-6-trifluoromethyl (6-nitro) benzotriazoles, respectively, do not give, under normal coupling conditions, phosphorylation of the lactam functions in uracil or guanine. Reagent 3b (X = O) proved to be very convenient for the in situ formation of 3'-5'-phosphotriester linkage. The latter will be illustrated by the synthesis of several RNA fragments.