Expression in mammalian cells of a gene fromStreptomyces albonigerconferring puromycin resistance | Zendy

Jesús Vara | Zendy; Agustı́n Portela | Zendy; Juan Ortı́n | Zendy; Antonio Moreno Jiménez | Zendy

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Expression in mammalian cells of a gene fromStreptomyces albonigerconferring puromycin resistance

Author(s) -

Jesús Vara,

Agustı́n Portela,

Juan Ortı́n,

Antonio Moreno Jiménez

Publication year - 1986

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/14.11.4617

Subject(s) - biology , puromycin , gene , gene expression , genetics , streptomyces , microbiology and biotechnology , bacteria , protein biosynthesis

The gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger has been cloned next to the SV40 early promoter in a mammalian cells-Escherichia coli shuttle vector. When this construction was introduced into VERO cells it expressed the relevant enzymic activity. Moreover, the puromycin N-acetyl transferase gene has been used as a dominant marker for the selection of transformed mammalian cells able to grow in the presence of the antibiotic.

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