Open AccessNucleotide sequence and transcriptional analysis of theE. coli ushA gene, encoding periplasmic UDP-sugar hydrolase (5'-nucleotidase): regulation of theushAgene, and the signal sequence of its encoded protein product
Author(s) -
Dennis M. Burns,
Ifor R. Beacham
Publication year - 1986
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/14.10.4325
Subject(s) - biology , gene , nucleic acid sequence , primer extension , signal peptide , peptide sequence , microbiology and biotechnology , hydrolase , sequence analysis , genetics , consensus sequence , nucleotide , biochemistry , enzyme
The DNA sequence of the ushA gene, encoding UDP-sugar hydrolase (5'-nucleotidase), has been determined. The amino-terminal sequence encodes a signal peptide whose predicted processing site is confirmed by N-terminal amino acid analysis of purified mature UshA protein. The signal sequence contains a concentration of rare codons in comparison with the mature sequence. The origins of transcription from the ushA promoter have been determined, using primer extension. Three transcripts, originating within a 6 bp region, were identified and might be related to three overlapping potential -10 hexamers in the ushA promoter region. There was a discernable change in the relative proportion of these transcripts during growth-phase regulation of the ushA gene.