Nucleotide sequence and transcriptional analysis of theE. coli ushA gene, encoding periplasmic UDP-sugar hydrolase (5'-nucleotidase): regulation of theushAgene, and the signal sequence of its encoded protein product | Zendy

Dennis M. Burns | Zendy; Ifor R. Beacham | Zendy

Open Access

Nucleotide sequence and transcriptional analysis of theE. coli ushA gene, encoding periplasmic UDP-sugar hydrolase (5'-nucleotidase): regulation of theushAgene, and the signal sequence of its encoded protein product

Author(s) -

Dennis M. Burns,

Ifor R. Beacham

Publication year - 1986

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/14.10.4325

Subject(s) - biology , gene , nucleic acid sequence , primer extension , signal peptide , peptide sequence , microbiology and biotechnology , hydrolase , sequence analysis , genetics , consensus sequence , nucleotide , biochemistry , enzyme

The DNA sequence of the ushA gene, encoding UDP-sugar hydrolase (5'-nucleotidase), has been determined. The amino-terminal sequence encodes a signal peptide whose predicted processing site is confirmed by N-terminal amino acid analysis of purified mature UshA protein. The signal sequence contains a concentration of rare codons in comparison with the mature sequence. The origins of transcription from the ushA promoter have been determined, using primer extension. Three transcripts, originating within a 6 bp region, were identified and might be related to three overlapping potential -10 hexamers in the ushA promoter region. There was a discernable change in the relative proportion of these transcripts during growth-phase regulation of the ushA gene.

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