Open AccessInfluence of uracil defect on DNA structure:1H NMR invastigation 500 MHz
Author(s) -
Anne-Marie Delort,
JeanMichel Neumann,
D. Molko,
Martine Hervé,
R. Téoule,
S. Tran Dinh
Publication year - 1985
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/13.9.3343
Subject(s) - glycosidic bond , uracil , two dimensional nuclear magnetic resonance spectroscopy , crystallography , oligonucleotide , dna , nuclear magnetic resonance spectroscopy , nuclear magnetic resonance , stereochemistry , biology , chemistry , physics , biochemistry , enzyme
The local structure of two self complementary oligonucleotides d(GTAC-GTAC) and d(GTACGUAC) which differ only by the presence of uracil, not a normal component of DNA, have been investigated by 1H NMR at 500 MHz. The two octamers exhibit the same thermodynamical constants (t 1/2, delta H), their exchangeable protons broaden and disappear at the same temperature. The T-U substitution did not induce any significant changes on non exchangeable protons resonances from 2-D COSY and 2-D NOESY experiments. So the two octamers exhibit the same global structure. The only variation was detected by 1D NOE measurements: the base orientations around the N glycosidic bonds (chi angles) are different.