Open AccessMolecular cloning and characterization of a gene coding for methanol oxidase inHansenula polymorpha
Author(s) -
A.M. Ledeboer,
Luppo Edens,
Jaap Maat,
Chris J. Visser,
J. Wil Bos,
C. Theo Verrips,
Zbigniew A. Janowicz,
Michael Eckart,
Rainer Roggenkamp,
Cornelis P. Hollenberg
Publication year - 1985
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/13.9.3063
Subject(s) - biology , nucleic acid sequence , gene , saccharomyces cerevisiae , coding region , yeast , genetics , structural gene , molecular cloning , cloning (programming) , peptide sequence , signal peptide , biochemistry , peroxisome , microbiology and biotechnology , escherichia coli , computer science , programming language
The structural gene and the regulatory DNA sequence of the yeast Hansenula polymorpha methanol oxidase have been isolated. According to the nucleotide sequence data obtained, the structural gene encodes a 664 amino acids long protein, contains no intervening sequences, and the 5'- and 3'-non-coding region contains several sequences implicated in transcription initiation and termination in the yeast Saccharomyces cerevisiae. Although the methanol oxidase is translocated to the peroxisomes, no cleavable signal sequence was found at the N-terminus of the protein.