Cloning and charactesization of theDASgene encoding the major methanol assimilatory enzyme from the methylotrophic yeastHansenula polymorpha | Zendy

Zbigniew A. Janowicz | Zendy; Michael R. Eckart | Zendy; Christel Drewke | Zendy; Rainer Roggenkamp | Zendy; Cornelis P. Hollenberg | Zendy; J. Maat | Zendy; A.M. Ledeboer | Zendy; Chris J. Visser | Zendy; C. Theo Verrips | Zendy

Open Access

Cloning and charactesization of theDASgene encoding the major methanol assimilatory enzyme from the methylotrophic yeastHansenula polymorpha

Author(s) -

Zbigniew A. Janowicz,

Michael R. Eckart,

Christel Drewke,

Rainer Roggenkamp,

Cornelis P. Hollenberg,

J. Maat,

A.M. Ledeboer,

Chris J. Visser,

C. Theo Verrips

Publication year - 1985

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/13.9.3043

Subject(s) - biology , gene , biochemistry , yeast , nucleic acid sequence , amino acid , saccharomyces cerevisiae , molecular cloning , cloning (programming) , microbiology and biotechnology , peptide sequence , coding region , enzyme , programming language , computer science

A gene library from the methanol utilizing yeast Hansenula polymorpha, constructed in a lambda Charon4A vector, was used to clone the gene encoding a key methanol assimilating enzyme, dihydroxyacetone synthase (DHAS) by differential plaque hybridization. The nucleotide sequence of the 2106 bp structural gene and the 5' and 3' non-coding regions was determined. The deduced amino acid sequence of the protein is in agreement with the apparent molecular weight and amino acid composition of the purified protein. The codon bias is not so pronounced as in some Saccharomyces cerevisiae genes.

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