Open AccessOrganization ofPlasmodium falciparum genome: 1. Evidence for a highly repeated DNA sequence
Author(s) -
Ramareddy V. Guntaka,
Siddarame Gowda,
Arepalli S. Rao,
Theodore J. Green
Publication year - 1985
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/13.6.1965
Subject(s) - biology , plasmodium falciparum , pbr322 , genome , plasmid , dna , repeated sequence , genetics , genomic organization , nucleic acid thermodynamics , nucleic acid , virology , dna sequencing , microbiology and biotechnology , genomic dna , gene , base sequence , malaria , immunology
Plasmodium falciparum DNA, isolated from the merozoite stage, was cleaved with HindIII and cloned in pBR322 and lambda L47.1 vectors. Plasmid clones containing 13.4, 7.0, 4.3, 4.1 and 1.5 kb inserts were characterized in some detail. The inserts contain several repeating units of smaller size. Nucleic acid hybridization studies showed that the repeat element is present in the Plasmodium DNA at a very high copy number and appears to be distributed widely throughout the genome.