Open AccessAnalysis of regulatory sequence upstream of theE. coli uvrBgene; involvement of the DnaA protein
Author(s) -
E.A. van den Berg,
Ruud H. Geerse,
Johan Memelink,
Roel A. L. Bovenberg,
F.A. Magnée,
Pieter van de Putte
Publication year - 1985
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/13.6.1829
Subject(s) - dnaa , biology , plasmid , gene , genetics , upstream activating sequence , regulatory sequence , homology (biology) , microbiology and biotechnology , dna , promoter , regulation of gene expression , gene expression , origin of replication
A region located upstream of the uvrB promoters P1 and P2 was found to cause high plasmid loss when cloned in multicopy vectors. Two sequence elements responsible for this phenomenon were identified by mapping of spontaneous mutations that restore plasmid maintenance: a sequence known to have in vitro promoter activity and a partially overlapping sequence that shows extensive homology to recognition sites for the DnaA protein. Accordingly alterations in the level of DnaA protein in vivo were found to affect the extent of plasmid loss. A possible role for interaction of the DnaA protein with the region of interest is discussed in relation to regulation of uvrB expression.