Open AccessrDNA inLocusta, migratoriais very variable: two introns and extensive restriction site polymorphisms in the spacer
Author(s) -
Mireille Schäfer,
Werner Kunz
Publication year - 1985
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/13.4.1251
Subject(s) - biology , intron , restriction site , genetics , restriction map , gene , restriction fragment , spacer dna , nuclease , restriction fragment length polymorphism , microbiology and biotechnology , dna , ribosomal dna , terminal restriction fragment length polymorphism , restriction enzyme , nucleic acid sequence , polymerase chain reaction , phylogenetics
Cloned ribosomal DNA (rDNA) of Locusta migratoria was analyzed by restriction site mapping and SI nuclease experiments. The repeat unit is 18 kb long. The nontranscribed spacer region (NTS) is very large (11 kb) and homogeneous in length, but many of the restriction sites are heterogeneous among the repeat units. Two introns of different length were found at different positions in the 28S gene. They are present in less than 5% of the genes.