Open AccessAlu sequences transcription inX. laevisoocytes: nuclear-cytoplasmic partitioning and evidence for 3′ end processing reactions
Author(s) -
Elda Perlino,
Giacomo Paonessa,
Gennaro Ciliberto
Publication year - 1985
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/13.23.8359
Subject(s) - biology , alu element , genetics , xenopus , transcription (linguistics) , cell nucleus , nucleus , rna polymerase iii , microbiology and biotechnology , gene , rna , genome , human genome , linguistics , philosophy , rna polymerase
A large Alu-family cluster in the 5' flanking region of a human alpha 1-acid glycoprotein gene has been identified and sequenced. Individual members microinjected into X. laevis oocytes are transcribed only when canonical box A and B components of the split pol III promoter are present. Alu transcripts accumulate in the nucleus. An unusually short Alu transcript, able to assume a stable secondary structure, undergoes a 3' end processing reaction similar to the one required for tRNA 3' end maturation.