Open Accessα-Amanitin insensitive transcription of the human η-globin gene
Author(s) -
George Kollias,
Constantin E. Sékeris,
Frank Grosveld
Publication year - 1985
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/13.22.7993
Subject(s) - biology , transcription (linguistics) , rna polymerase ii , general transcription factor , microbiology and biotechnology , transcription factor ii d , transcription factor ii f , rna polymerase , e box , transcription preinitiation complex , transcription factor ii b , response element , rna polymerase ii holoenzyme , transcription bubble , gene expression , promoter , polymerase , gene , rna , genetics , philosophy , linguistics
In vitro transcription was used to show that RNA polymerase III is responsible for the initiation of transcription at a position 200 bp upstream from the epsilon-globin major cap site. High levels of -200 transcription interferes with the RNA polymerase II major cap site transcription. Using DNA mediated transient expression, the ratio of -200 to +1 transcription can be modulated by either the direction of replication or the presence of an enhancing element in the vector. We suggest that this heterogeneous usage of cap sites is not related to epsilon-globin gene transcription in vivo, but is instead the result of a combination of factors inherent to transient expression experiments.