Nucleotide sequence ofCandida pelliculosaβ-glucosidase gene | Zendy

Chie Kohchi | Zendy; Akio Tohe | Zendy

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Nucleotide sequence ofCandida pelliculosaβ-glucosidase gene

Author(s) -

Chie Kohchi,

Akio Tohe

Publication year - 1985

Publication title -

nucleic acids research

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/13.17.6273

Subject(s) - biology , open reading frame , codon usage bias , gene , nucleic acid sequence , saccharomyces cerevisiae , genetics , peptide sequence , heterologous expression , nucleotide , signal peptide , start codon , stop codon , microbiology and biotechnology , dna , recombinant dna , genome

The nucleotide sequence of the DNA fragment containing the beta-glucosidase gene of Candida pelliculosa was determined. Analysis of the sequence revealed three open reading frames which could encode 65,825, and 412 amino acid residues. The presence of the second frame was found to be sufficient for the expression of the beta-glucosidase gene in a heterologous host Saccharomyces cerevisiae. Putative protein encoded by this gene had hydrophobic amino acids, resembling a signal peptide, at its N-terminal region and 19 potential glycosylation sites. Codon usage of Candida genes had the similar pattern shown in S.cerevisiae. Codon bias of the beta-glucosidase gene of Candida was relatively low, compared with that of the highly expressed genes of S. cerevisiae.

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