Open AccessA convenient technique to compare the efficiency of promoters inEscherichia coli
Author(s) -
Dominique Vidal-Ingigliardi,
Olivier Raibaud
Publication year - 1985
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/13.16.5919
Subject(s) - promoter , biology , operon , plasmid , escherichia coli , genetics , insert (composites) , gene , microbiology and biotechnology , computational biology , gene expression , mechanical engineering , engineering
We describe a technique which allows one to insert any promoter in front of the chromosomal malPQ operon. This can be done easily by using only one plasmid, one strain, and two simple selections. Properties of the final chromosomal fusion are such that the level of amylomaltase, the product of the malQ gene, measures quantitatively the efficiency of the inserted promoter. This method was utilized to compare the efficiency of four well-known promoters: lacZp, trp, tac, lambdaPR and three malT activated promoters: malPp, malkP and malEp.