Open AccessComplete nucleotide sequence of the lipase gene fromStaphylococcus hyicuscloned inStaphylococcus carnosus
Author(s) -
Friedrich Götz,
Felix Popp,
Edward D. Korn,
Karl Heinz Schleifer
Publication year - 1985
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/13.16.5895
Subject(s) - biology , nucleic acid sequence , lipase , gene , open reading frame , microbiology and biotechnology , escherichia coli , structural gene , genetics , peptide sequence , biochemistry , enzyme
The lipase gene from Staphylococcus hyicus subsp. hyicus was cloned in Staphylococcus carnosus and Escherichia coli. In both host organisms the lipase gene is expressed and the enzyme is released to the medium. The cloned DNA insert is 2.5 kb in length and DNA sequencing has revealed the location of the gene, the ribosomal binding site and the presence of a typical signal sequence. The open reading frame comprises 1923 nucleotides and gives a preprotein of 641 amino acids with a predicted Mr of 71.382. At the 3' end of the structural gene there are three consecutive stop codons and there is also a transcriptional termination signal.