Open AccessThe nucleotide sequence of thetnpAgene completes the sequence of thePseudomonastransposon Tn501
Author(s) -
Nigel L. Brown,
Joseph N. Winnie,
David C. Fritzinger,
R. David Pridmore
Publication year - 1985
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/13.15.5657
Subject(s) - biology , transposase , genetics , transposable element , open reading frame , nucleic acid sequence , gene , peptide sequence , sleeping beauty transposon system , sequence analysis , reading frame , inverted repeat , microbiology and biotechnology , genome
The nucleotide sequence of the gene (tnpA) which codes for the transposase of transposon Tn501 has been determined. It contains an open reading frame for a polypeptide of Mr = 111,500, which terminates within the inverted repeat sequence of the transposon. The reading frame would be transcribed in the same direction as the mercury-resistance genes and the tnpR gene. The amino acid sequence predicted from this reading frame shows 32% identity with that of the transposase of the related transposon Tn3. The C-terminal regions of these two polypeptides show slightly greater homology than the N-terminal regions when conservative amino acid substitutions are considered. With this sequence determination, the nucleotide sequence of Tn501 is fully defined. The main features of the sequence are briefly presented.