Alkali-labile structures linked to the 5′ ends ofBacillus subtilisshort DNA chains | Zendy

Hachiro Yasuda | Zendy; Tuneko Okazaki | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Alkali-labile structures linked to the 5′ ends ofBacillus subtilisshort DNA chains

Author(s) -

Hachiro Yasuda,

Tuneko Okazaki

Publication year - 1985

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/13.13.4953

Subject(s) - biology , bacillus subtilis , dna , oligonucleotide , primer (cosmetics) , primase , rna , microbiology and biotechnology , polymerase , biochemistry , genetics , reverse transcriptase , bacteria , gene , chemistry , organic chemistry

Alkali-labile portion covalently linked to the 5' ends of Bacillus subtilis short DNA chains, the putative primer RNA for discontinuous DNA synthesis, was isolated and analyzed using a temperature sensitive DNA polymerase I mutant, which accumulates nascent DNA fragments at a restrictive temperature. A novel oligonucleotide structure as well as mono- to triribonucleotide stretches were isolated at the 5' end of the short DNA chains. A structure for the novel oligonucleotide is proposed to be p5' X3' pp5' rN, where X represents unidentified nucleoside with a peculiar property. Possible metabolic relationship between these molecules and primer RNA has been discussed.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research