Open AccessAlkali-labile structures linked to the 5′ ends ofBacillus subtilisshort DNA chains
Author(s) -
Hachiro Yasuda,
Tuneko Okazaki
Publication year - 1985
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/13.13.4953
Subject(s) - biology , bacillus subtilis , dna , oligonucleotide , primer (cosmetics) , primase , rna , microbiology and biotechnology , polymerase , biochemistry , genetics , reverse transcriptase , bacteria , gene , chemistry , organic chemistry
Alkali-labile portion covalently linked to the 5' ends of Bacillus subtilis short DNA chains, the putative primer RNA for discontinuous DNA synthesis, was isolated and analyzed using a temperature sensitive DNA polymerase I mutant, which accumulates nascent DNA fragments at a restrictive temperature. A novel oligonucleotide structure as well as mono- to triribonucleotide stretches were isolated at the 5' end of the short DNA chains. A structure for the novel oligonucleotide is proposed to be p5' X3' pp5' rN, where X represents unidentified nucleoside with a peculiar property. Possible metabolic relationship between these molecules and primer RNA has been discussed.