Open AccessNucteotide sequences of bovine αS1- andx-casein cDNAs
Author(s) -
A. Francis Stewart,
Ian M. Willis,
A.F. Mackinlay
Publication year - 1984
Publication title -
nucleic acids research
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/12.9.3895
Subject(s) - biology , complementary dna , exon , nucleic acid sequence , coding region , messenger rna , casein , genetics , gene , point mutation , microbiology and biotechnology , sequence (biology) , peptide sequence , shuffling , mutation , biochemistry , computer science , programming language
The nucleotide sequences corresponding to bovine alpha S1- and kappa-casein mRNAs are presented. An unusual alpha S1-casein cDNA has been characterised whose 5' end commences upstream from its putative TATA box. The alpha S1-casein mRNA is compared to rat alpha-casein mRNA and two components of divergence are identified. Firstly, the two sequences have diverged at a high point mutation rate and the rate of amino acid replacement by this mechanism is at least as great as the rate of divergence of any other part of the mRNAs. Secondly, the protein coding sequence has been subjected to several insertion/deletion events, one of which may be an example of exon shuffling . The kappa-casein mRNA sequence verifies the proposition that it has arisen from a different ancestral gene to the other caseins.
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