Open AccessThe genes for the ribosomal proteins S12 and S7 are clustered with the gene for the EF-Tu protein on the chloroplast genome ofEuglena gracilis
Author(s) -
Paul-Etienne Montandon,
Erhard Stutz
Publication year - 1984
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/12.6.2851
Subject(s) - biology , chloroplast dna , ribosomal protein , euglena gracilis , genetics , gene , operon , ribosomal rna , genome , chloroplast , nucleic acid sequence , microbiology and biotechnology , ribosome , escherichia coli , rna
We characterize a DNA segment of the Euglena gracilis chloroplast DNA fragment Eco . N by nucleotide sequencing and S1 nuclease analysis. We show that this region, which is upstream of the previously sequenced tuf A gene, contains the genes for the ribosomal proteins S12 and S7. The gene arrangement is 5'-rps 12-80 bp spacer-rps 7-174 bp spacer-tuf A, somewhat similar to the str operon of E. coli. The chloroplast S12 and S7 proteins contain 124 and 155 aminoacids, respectively, and are to 68% and 38% homologous with the corresponding E. coli proteins. The region is transcribed into a distronic mRNA of about 1.1 to 1.2 kb. The rps 12 and rps 7 genes, contrary to the tuf A gene, are not split.