Open AccessThe 11S rat seminal vesicle mRNA directs thein vitrosynthesis of two precursors of the major secretory protein IV
Author(s) -
Salvatore Metafora,
John Guardiola,
Giacomo Paonessa,
Paolo Abrescia
Publication year - 1984
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/12.4.1901
Subject(s) - biology , messenger rna , translation (biology) , protein biosynthesis , microbiology and biotechnology , vesicle , in vitro , gel electrophoresis , polyacrylamide gel electrophoresis , biochemistry , gene , membrane , enzyme
The 11s mRNA extracted from the rat seminal vesicles directs the synthesis of two different precursors of the major secretory protein RSV-IV. These two precursors are not interconvertible and seemingly originate from different translational events. Sucrose gradients, polyacrylamide gel electrophoresis and positive hybridization translation experiments do not allow the separation of the two putatively different mRNAs. It is concluded that the two RSV-IV precursors either derive from two extremely similar, but physically not separable mRNA species, or from two different modes of translation of the same mRNA molecule.