Open Access2′, 3′-Dideoxy-3′ amlnonudeo 5′ .triphosphates are the terminators of DNA synthesis catalyzed by DNA polymerases
Author(s) -
Zurab G. Chidgeavadze,
Robert Sh. Beabealashvilli,
Alexey Atrazhev,
Marina K. Kukhanova,
Alex Azhayev,
Alexander A. Krayevsky
Publication year - 1984
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/12.3.1671
Subject(s) - biology , dna polymerase , dna polymerase ii , primer (cosmetics) , dna clamp , primase , polymerase , biochemistry , dna , microbiology and biotechnology , dna polymerase i , thymine , guanine , cytosine , dna replication , nucleotide , dna synthesis , polymerase chain reaction , reverse transcriptase , gene , chemistry , organic chemistry
It is shown that 2',3'-dideoxy-3'-aminonucleoside 5'-triphosphates with adenine, guanine, cytosine and thymine bases are effective inhibitors of DNA polymerase I, calf thymus DNA polymerase alpha and rat liver DNA polymerase beta. The effect of the above-mentioned compounds is markedly higher than corresponding action of the well-known DNA synthesis inhibitors arabinonucleoside 5'-triphosphates and 2',3'-dideoxynucleoside 5'-triphosphates. 2',3'-dideoxy-3'-aminonucleoside 5'-monophosphate residues incorporate into the 3'-terminus of the primer and terminate the DNA chain elongation. The possibility of using 2',3'-dideoxy-3'-aminonucleoside 5'-triphosphates as terminators for DNA sequencing by the polymerization method is demonstrated.