Expression of Ty-lacZfusions inSaccharomyces cerevisiae | Zendy

Benjamin A. Bowen | Zendy; Alexandra M. Fulton | Zendy; Mick F. Tuite | Zendy; Susan M. Kingsman | Zendy; Alan J. Kingsman | Zendy

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Expression of Ty-lacZfusions inSaccharomyces cerevisiae

Author(s) -

Benjamin A. Bowen,

Alexandra M. Fulton,

Mick F. Tuite,

Susan M. Kingsman,

Alan J. Kingsman

Publication year - 1984

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/12.3.1627

Subject(s) - biology , open reading frame , lac operon , saccharomyces cerevisiae , genetics , translation (biology) , nucleic acid sequence , nucleotide , yeast , microbiology and biotechnology , gene , plasmid , messenger rna , peptide sequence

We have determined the nucleotide sequence of about 520 bp spanning the 5' delta regions (Figure 1) of two Tyl elements. There is an open reading frame running out of the deltas for at least 180 nucleotides into the internal region of each element. The functional significance of these open reading frames has been tested by fusing them to a defective E.coli lacZ gene. Expression of B-galactosidase in yeast transformants containing these fusions shows that Tyl elements contain functional translation signals.

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