Open AccessNucleotide sequence ofRhizobium melilotinodulation genes
Author(s) -
I. Török,
Éva Kondorosi,
Tomasz Stępkowski,
János Pósfai,
Ádám Kondorosi
Publication year - 1984
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/12.24.9509
Subject(s) - biology , rhizobium , rhizobium leguminosarum , nucleic acid sequence , genetics , gene , homology (biology) , coding region , nucleotide , rhizobiaceae , bacteria , symbiosis
A Rhizobium meliloti DNA region, determining nodulation functions common in different Rhizobium species, has been delimited by directed Tn5 mutagenesis and its nucleotide sequence has been determined. The sequence data indicates three large open reading frames with the same polarity coding for three proteins of 196, 217 and 402 (or 426) amino acid residues, respectively. We suggest the existence of three nod genes on this region, which were designated as nodA, B and C, respectively. Comparison of the R. meliloti nodA, B, C nucleotide and amino acid sequences with those from R. leguminosarum, as reported in the accompanying paper, shows 69-72% homology, clearly demonstrating the high degree of conservation of common nod genes in these Rhizobium species.