Open AccessComplete nucleotide sequence of theE. coliglutathione synthetase gsh-II
Author(s) -
Hiroshi Gushima,
Shuhay Yasuda,
Eiichi Soeda,
Masami Yokota,
Masatoshi Kondo,
Akira Kimura
Publication year - 1984
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/12.24.9299
Subject(s) - biology , nucleic acid sequence , open reading frame , coding region , gene , peptide sequence , microbiology and biotechnology , start codon , glutathione , genetics , amino acid , nucleotide , biochemistry , reading frame , conserved sequence , enzyme
The nucleotide sequence of the cloned DNA, 1,478 bp in length coding for glutathione synthetase (GSH-II) of E. coli B has been determined. Amino acid and nucleotide sequence analyses have assigned the open reading frame for GSH-II, starting with the ATG near its 5' terminus. The molecular weight calculated from the predicted amino acid sequence is 35,559 daltons, being in good agreement with that of a GSH-II subunit estimated by the SDS-PAGE method. Several signal sequences conserved in the promoter regions of E. coli were found in the non-coding regions of the gsh-II gene. They include the Shine-Dalgarno sequence, the Pribnow box and the sequence conserved in the "-35 region" with a preferable spacing from each other for an efficient transcription. Downstream from the termination codon, the inverted repeat sequences were present, followed by 6 successive T's. These structural features found in the non-coding regions have suggested to be involved in regulatory functions for the gsh-II gene expression.