Open AccessSynthesis of phosphorothloate-contaming DNA fragments by a modified bydroxybenzotriazole phospbotriester approach
Author(s) -
J. E. Marugg,
Caroline Bergh,
M. Tromp,
G.A. van der Marel,
W. J. Van Zoest,
J. H. van Boom
Publication year - 1984
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/12.23.9095
Subject(s) - biology , dna , combinatorial chemistry , dna synthesis , computational biology , microbiology and biotechnology , genetics , chemistry
The phosphorothioylating agent which was obtained by treating 2,5-dichlorophenyl phosphorodichloridothioate with 1-hydroxy-6-nitrobenzotriazole proved to be very effective for the synthesis in solution and on a solid support of phosphorothioate-containing DNA fragments.